A Novel Modular Bioreactor to In Vitro Study the Hepatic Sinusoid
نویسندگان
چکیده
We describe a unique, versatile bioreactor consisting of two plates and a modified commercial porous membrane suitable for in vitro analysis of the liver sinusoid. The modular bioreactor allows i) excellent control of the cell seeding process; ii) cell culture under controlled shear stress stimulus, and; iii) individual analysis of each cell type upon completion of the experiment. The advantages of the bioreactor detailed here are derived from the modification of a commercial porous membrane with an elastomeric wall specifically moulded in order to define the cell culture area, to act as a gasket that will fit into the bioreactor, and to provide improved mechanical robustness. The device presented herein has been designed to simulate the in vivo organization of a liver sinusoid and tested by co-culturing endothelial cells (EC) and hepatic stellate cells (HSC). The results show both an optimal morphology of the endothelial cells as well as an improvement in the phenotype of stellate cells, most probably due to paracrine factors released from endothelial cells. This device is proposed as a versatile, easy-to-use co-culture system that can be applied to biomedical research of vascular systems, including the liver.
منابع مشابه
In vitro zonation and toxicity in a hepatocyte bioreactor.
The complex architecture of the liver is intertwined with its response to xenobiotic compounds. In particular, hepatocyte subpopulations are distributed along the sinusoid in zones 1 to 3, leading to prototypical "periportal" and "centrilobular" patterns of cell death in response to a toxic insult. In vitro models that more closely represent these zones of sub-specialization may therefore be va...
متن کاملTreatment of Real Paper-Recycling Wastewater in a Novel Hybrid Airlift Membrane Bioreactor (HAMBR) for Simultaneous Removal of Organic Matter and Nutrients
In this study, a novel integrated Hybrid Airlift Membrane Bioreactor (HAMBR) composed of oxic, anoxic, and anaerobic zones was developed to simultaneously remove organic matter and nitrogen from real paper-recycling wastewater. The removal efficiencies of Chemical Oxygen Demand (COD), ammonium, nitrite, nitrate and Total Nitrogen (TN) for permeate and supernatant were in the range of ...
متن کاملHigh-Resolution Micro-CT for Morphologic and Quantitative Assessment of the Sinusoid in Human Cavernous Hemangioma of the Liver
Hepatic sinusoid plays a vital role in human cavernous hemangioma of the liver (CHL), and its morphologic investigation facilitates the understanding of microcirculation mechanism and pathological change of CHL. However, precise anatomical view of the hepatic sinusoid has been limited by the resolution and contrast available from existing imaging techniques. While liver biopsy has traditionally...
متن کاملEFFICIENT SIMULATION FOR OPTIMIZATION OF TOPOLOGY, SHAPE AND SIZE OF MODULAR TRUSS STRUCTURES
The prevalent strategy in the topology optimization phase is to select a subset of members existing in an excessively connected truss, called Ground Structure, such that the overall weight or cost is minimized. Although finding a good topology significantly reduces the overall cost, excessive growth of the size of topology space combined with existence of varied types of design variables challe...
متن کاملA Novel Hybrid-Excited Modular Variable Reluctance Motor for Electric Vehicle Applications: Analysis, Comparison, and Implementation
A variable reluctance machine (VRM) has been proven to be an outstanding candidate for electric vehicle (EV) applications. This paper introduces a new double-stator, 12/14/12-pole three-phase hybrid-excited modular variable reluctance machine (MVRM) for EV applications. In order to demonstrate the superiorities of the proposed structure, the static torque characteristics and dynamic performance...
متن کامل